Fig. 3
From: pH-mediated upregulation of AQP1 gene expression through the Spi-B transcription factor
Transcription factors interacting with the enhancer region of the AQP1 gene. a Luciferase activity associated with the region between − 2.3k and 0 bp in AQP1 in HEK-293T cells transfected with small interfering RNAs (siRNAs) against EGR1, FOXL1, KLF5, NFIC, RFX5, SP1, THAP1, or SPIB or with a negative control (NC). b AQP1 mRNA levels in HEK-293T cells after SPIB knockdown and treatment at pH 7 and 8 were assessed with real-time PCR. The data represent the mean ± SEM. ns no statistical significance; ***P < 0.001