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Table 3 Summary of studies using formalin-fixed human renal biopsies for glomerular micro-dissection and gene expression analysis of glomerular mRNA

From: Recommendations for mRNA analysis of micro-dissected glomerular tufts from paraffin-embedded human kidney biopsy samples

First author Sections cut in (µm) Staining method Number of micro-dissected glomerular transections RNA extraction RNA purity (A260/A280 ratio) Total RNA quantity used for cDNA synthesis Preamplification Glomerular reference gene(s) Lit.
Cohen 5 Hemalaun 20–50 Phenol–chloroform nd nd None GAPDH [10]
Fries 5 None 10–25 Purescript RNA isolation kit (Gentra, Minneapolis, MN) nd nd None ACTB [12]
Schmid 5 Hemalaun 20–50 RNeasy-Mini nd nd None GAPDH and 18 S rRNA [11]
Landolt 10 HE 100 Various were compared 1.1–1.9 54–138 ng Only pre-analytical steps were performed [9]
Bao Not given None 50 mirVana miRNA Isolation Kit nd nd None ACTB [13]
Bockmeyer 2 Comparing no staining and Hemalaun Comparing 20–300 Phenol–chloroform 0.9 to 2.3 157–282 ng Yes PGK1, PPIA This study
  1. nd not determined