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Table 2 TaqMan assay identity numbers, amplicon size and characteristics of analyzed candidate reference and target transcripts

From: Recommendations for mRNA analysis of micro-dissected glomerular tufts from paraffin-embedded human kidney biopsy samples

Gene symbol (full name) TaqMan assay identity number Amplicon size Chromosomal location Function
PGK1 (Phosphoglycerate kinase 1) Hs00943178_g1 73 Xq21.1 Kinase that catalyzes the conversion of phosphoglycerates; also involved in tumor angiogenesis
POLR2A (RNA polymerase II subunit A) Hs00172187_m1 61 17p13.1 Synthesizing mRNA
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) Hs99999905_m1 122 12p13.31 Important in carbohydrate metabolism
GUSB (Glucuronidase, beta) Hs99999908_m1 81 7q11.21 Hydrolases glycosaminoglycans
PPIA (Peptidylprolyl isomerase A) Hs99999904_m1 98 7p13 Catalyzes the cis–trans isomerization of peptide bonds in oligopeptides. Possible role in cyclosporine A-mediated immunosuppression
ACTB (Beta-Aktin) Hs01060665_g1 63 7p22.1 Nonmuscle cytoskeletal actin that is ubiquitously expressed
Eukaryotic 18S rRNA Hs03003631_g1 69 12 Catalyzes the synthesis of peptides and proteins
B2M (Beta-2 microglobulin) Hs00187842_m1 64 15q21.1 Protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells
HPRT1 (Hypoxanthine phosphoribosyltransferase 1) Hs02800695_m1 82 Xq26.2-q26.3 Plays a central role in the generation of purine nucleotides
RPLPO (Ribosomal protein lateral stalk subunit P0) Hs00420895_gH 76 12q24.23 Encodes a ribosomal protein that is a component of the 60S subunit
TBP (TATA-box binding protein) Hs00427620_m1 91 6q27 Coordinates Initiation of transcription by RNA polymerase II 
HMBS (Hydroxymethylbilane synthase) Hs00609297_m1 64 11q23.3 Encodes a member of the hydroxymethylbilane synthase superfamily
WT1 (Wilms tumor 1) Hs01103751_m1 72 11p13 Development of the urogenital system
PAX2 (Paired box 2) Hs00240858_m1 57 10q24.31 target of tumor suppressor gene WT1
PTPRO (Protein tyrosine phosphatase, receptor type O, alias GLEPP1) Hs00958189_m1 91 12p12.3 Regulation of osteoclast production and activity, inhibition of cell proliferation and facilitation of apoptosis
ADAMTS13 (A disintegrin and metalloproteinase with thrombospondin type 1 motives 13) Hs00260148_m1 59 9q34.2 Cleaves von Willebrand factor