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Table 2 TaqMan assay identity numbers, amplicon size and characteristics of analyzed candidate reference and target transcripts
| Gene symbol (full name) | TaqMan assay identity number | Amplicon size | Chromosomal location | Function |
|---|---|---|---|---|
| PGK1 (Phosphoglycerate kinase 1) | Hs00943178_g1 | 73 | Xq21.1 | Kinase that catalyzes the conversion of phosphoglycerates; also involved in tumor angiogenesis |
| POLR2A (RNA polymerase II subunit A) | Hs00172187_m1 | 61 | 17p13.1 | Synthesizing mRNA |
| GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) | Hs99999905_m1 | 122 | 12p13.31 | Important in carbohydrate metabolism |
| GUSB (Glucuronidase, beta) | Hs99999908_m1 | 81 | 7q11.21 | Hydrolases glycosaminoglycans |
| PPIA (Peptidylprolyl isomerase A) | Hs99999904_m1 | 98 | 7p13 | Catalyzes the cis–trans isomerization of peptide bonds in oligopeptides. Possible role in cyclosporine A-mediated immunosuppression |
| ACTB (Beta-Aktin) | Hs01060665_g1 | 63 | 7p22.1 | Nonmuscle cytoskeletal actin that is ubiquitously expressed |
| Eukaryotic 18S rRNA | Hs03003631_g1 | 69 | 12 | Catalyzes the synthesis of peptides and proteins |
| B2M (Beta-2 microglobulin) | Hs00187842_m1 | 64 | 15q21.1 | Protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells |
| HPRT1 (Hypoxanthine phosphoribosyltransferase 1) | Hs02800695_m1 | 82 | Xq26.2-q26.3 | Plays a central role in the generation of purine nucleotides |
| RPLPO (Ribosomal protein lateral stalk subunit P0) | Hs00420895_gH | 76 | 12q24.23 | Encodes a ribosomal protein that is a component of the 60S subunit |
| TBP (TATA-box binding protein) | Hs00427620_m1 | 91 | 6q27 | Coordinates Initiation of transcription by RNA polymerase II |
| HMBS (Hydroxymethylbilane synthase) | Hs00609297_m1 | 64 | 11q23.3 | Encodes a member of the hydroxymethylbilane synthase superfamily |
| WT1 (Wilms tumor 1) | Hs01103751_m1 | 72 | 11p13 | Development of the urogenital system |
| PAX2 (Paired box 2) | Hs00240858_m1 | 57 | 10q24.31 | target of tumor suppressor gene WT1 |
| PTPRO (Protein tyrosine phosphatase, receptor type O, alias GLEPP1) | Hs00958189_m1 | 91 | 12p12.3 | Regulation of osteoclast production and activity, inhibition of cell proliferation and facilitation of apoptosis |
| ADAMTS13 (A disintegrin and metalloproteinase with thrombospondin type 1 motives 13) | Hs00260148_m1 | 59 | 9q34.2 | Cleaves von Willebrand factor |
