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Table 2 TaqMan assay identity numbers, amplicon size and characteristics of analyzed candidate reference and target transcripts

From: Recommendations for mRNA analysis of micro-dissected glomerular tufts from paraffin-embedded human kidney biopsy samples

Gene symbol (full name)

TaqMan assay identity number

Amplicon size

Chromosomal location

Function

PGK1 (Phosphoglycerate kinase 1)

Hs00943178_g1

73

Xq21.1

Kinase that catalyzes the conversion of phosphoglycerates; also involved in tumor angiogenesis

POLR2A (RNA polymerase II subunit A)

Hs00172187_m1

61

17p13.1

Synthesizing mRNA

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase)

Hs99999905_m1

122

12p13.31

Important in carbohydrate metabolism

GUSB (Glucuronidase, beta)

Hs99999908_m1

81

7q11.21

Hydrolases glycosaminoglycans

PPIA (Peptidylprolyl isomerase A)

Hs99999904_m1

98

7p13

Catalyzes the cis–trans isomerization of peptide bonds in oligopeptides. Possible role in cyclosporine A-mediated immunosuppression

ACTB (Beta-Aktin)

Hs01060665_g1

63

7p22.1

Nonmuscle cytoskeletal actin that is ubiquitously expressed

Eukaryotic 18S rRNA

Hs03003631_g1

69

12

Catalyzes the synthesis of peptides and proteins

B2M (Beta-2 microglobulin)

Hs00187842_m1

64

15q21.1

Protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells

HPRT1 (Hypoxanthine phosphoribosyltransferase 1)

Hs02800695_m1

82

Xq26.2-q26.3

Plays a central role in the generation of purine nucleotides

RPLPO (Ribosomal protein lateral stalk subunit P0)

Hs00420895_gH

76

12q24.23

Encodes a ribosomal protein that is a component of the 60S subunit

TBP (TATA-box binding protein)

Hs00427620_m1

91

6q27

Coordinates Initiation of transcription by RNA polymerase II 

HMBS (Hydroxymethylbilane synthase)

Hs00609297_m1

64

11q23.3

Encodes a member of the hydroxymethylbilane synthase superfamily

WT1 (Wilms tumor 1)

Hs01103751_m1

72

11p13

Development of the urogenital system

PAX2 (Paired box 2)

Hs00240858_m1

57

10q24.31

target of tumor suppressor gene WT1

PTPRO (Protein tyrosine phosphatase, receptor type O, alias GLEPP1)

Hs00958189_m1

91

12p12.3

Regulation of osteoclast production and activity, inhibition of cell proliferation and facilitation of apoptosis

ADAMTS13 (A disintegrin and metalloproteinase with thrombospondin type 1 motives 13)

Hs00260148_m1

59

9q34.2

Cleaves von Willebrand factor