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Fig. 1 | BMC Molecular Biology

Fig. 1

From: Recommendations for mRNA analysis of micro-dissected glomerular tufts from paraffin-embedded human kidney biopsy samples

Fig. 1

Different resolution of defined glomerular structures in dependence on deparaffinization. Without deparaffinization (A, B), only coarse structures are visible. However, deparaffinization and a short hemalaun stain allow the detection of nuclei of PECs (green arrowheads) and endothelial (red arrowheads) cells (C, D). By contrast, globally (E, black arrow) or segmentally (F, black arrow) sclerosed glomeruli as well as scarred tubulo interstitium (E, black arrowhead) can easily be detected without deparaffinization. One glomerulus with open capillary loops is shown in comparison (F, green arrow). HE (G) and PAS (H, I) stains from consecutive sections of D–F are given. PALM/Zeiss laser technique and software, magnification ×400 or ×200, C, D short hemalaun stain

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