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Fig. 4 | BMC Molecular Biology

Fig. 4

From: Nutrient depletion and TOR inhibition induce 18S and 25S ribosomal RNAs resistant to a 5′-phosphate-dependent exonuclease in Candida albicans and other yeasts

Fig. 4

Incorporation of Terminator resistant 18S and 25S transcripts into ribosomes, and analysis of other yeasts for similar resistant rRNAs. a Agarose gel of RNA precipitated from isolated ribosomes from stationary phase cells. Numbers indicate fractions collected sequentially. Different elution fractions were combined prior to precipitation of RNA from isolated ribosomes from mid-log phase cells. Amount of Terminator digested RNA was the same as their undigested RNA pair. b SYBR gold stained gel containing RNA digested with Terminator (D) and undigested (U) isolated from mid log and stationary Candida albicans, Candida krusei, Schizosaccharomyces pombe and Saccharomyces cerevisiae

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