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Fig. 1 | BMC Molecular Biology

Fig. 1

From: An optimized rapid bisulfite conversion method with high recovery of cell-free DNA

Fig. 1

Binding sites of primers and probe used are underlined [23]. The top strand is the undeaminated DNA sequence. Bottom strand is the deaminated sequence. A solid line between the two strands illustrates no difference in sequence, a dotted line marks the positions of cytosine converted to uracil during deamination, and “+” marks the positions of CpG dinucleotides. Primer sequences can be found in Table 1. The universal primers, MLH1 UF and MLH1 R, contain Inosine at one position each

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