Table 2 Protocol for Cresyl violet staining of FFPE tissue sections

100% Xylene, bath 1

5 min

100% Xylene, bath 2

5 min

100% Ethanol

30 s

95% Ethanol

30 s

70% Ethanol

30 s

dH₂O

10 s

Cresyl violet (75% Ethanol with DEPC treated dH₂O, pH 8.0)

15 s

dH₂O

10 s

70% Ethanol

10 s

95% Ethanol, bath 1

10 s

95% Ethanol, bath 2

10 s

100% Ethanol, bath 1

30–60 s

100% Ethanol, bath 2

30–60 s