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Table 2 Protocol for Cresyl violet staining of FFPE tissue sections

From: An optimised protocol for isolation of RNA from small sections of laser-capture microdissected FFPE tissue amenable for next-generation sequencing

Cresyl violet staining procedure for FFPE tissue sections
100% Xylene, bath 1 5 min
100% Xylene, bath 2 5 min
100% Ethanol 30 s
95% Ethanol 30 s
70% Ethanol 30 s
dH2O 10 s
Cresyl violet (75% Ethanol with DEPC treated dH2O, pH 8.0) 15 s
dH2O 10 s
70% Ethanol 10 s
95% Ethanol, bath 1 10 s
95% Ethanol, bath 2 10 s
100% Ethanol, bath 1 30–60 s
100% Ethanol, bath 2 30–60 s