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Fig. 2 | BMC Molecular Biology

Fig. 2

From: An optimised protocol for isolation of RNA from small sections of laser-capture microdissected FFPE tissue amenable for next-generation sequencing

Fig. 2

Comparison of workflow of the ‘old’(classical) and ‘new’ protocol for RNA isolation from deparaffinised, stained and laser-capture microdissected FFPE tissue. a Workflow of the ‘old’ RNA isolation protocol, starting with proteinase K digestion of the sample. b Workflow of the ‘new’ isolation protocol, starting with a focused ultrasonication step to disrupt the tissue prior to proteinase K digestion. Values in brackets indicate the approximate timing for each step

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