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Fig. 7 | BMC Molecular Biology

Fig. 7

From: Splicing arrays reveal novel RBM10 targets, including SMN2 pre-mRNA

Fig. 7

SMN RNA and protein expression in a stable RBM10 KD MCF-7 subline. M300.5 was a stable MCF-7 subline expressing a scrambled shRNA, and M29/30.2 was a stable MCF-7 subline expressing two RBM10 exon 6-specific shRNAs. a, b RNA expression. a Lane 1 ladder, lane 2 end-point PCR of M300.5 control using GAPDH primers, lanes 3 and 4 a duplicate load of an end-point PCR of M300.5 using the SMN primers; lane 5 blank, lane 6 end-point PCR of M29/30.2 RBM10 KD using GAPDH primers, lanes 7 and 8 a duplicate load of an end-point PCR of M29/30.2 using the SMN primers. b RNA expression following transient transfection, as previously described [26], of a pcDNA3-based plasmid containing RBM10v1. c Protein expression. (i) One representative Western blot of SMN protein expression. (ii) % SMNFL, compared to SMNΔ7, from two biological replicates. Results were expressed as means and standard errors

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