From: Mitochondrial RNA processing in absence of tRNA punctuations in octocorals
Gene | 5′ RACE | Sequence | 3′ RACE | Sequence |
---|---|---|---|---|
A. Primers used in 5′ and 3′ RACE amplification | ||||
 COI | C1-839a | ATCATAGCATAGACCATACC |  |  |
C1-1144 | CATAGTGGAAGTGAGCTACTAC | Â | Â | |
 CytB–ND6 | CB-4128a | GCTCCCCAAAAGGACATTTGTC |  |  |
 ND3–ND4–mtMutS | N3-5602 | CACATTCATAGACCGACACTT | N4L-6071 | GCCATTATGGTTAACTATTAC |
M-6402 | ACGAAGCAACTTGTTCAATGG | M-6363 | ATTGAACAAGTTGCYTCGTTACTTG | |
M-6719 | CCGGGTTACTTTGTCCCTGTCCG | M-8714 | GCCCTCTCAATATGGCATTG | |
 |  | M-6655 | CAGCCATGAATGGGCATAG | |
 |  | M-8067 | GCATTAAGCGGGGCTATTGCGG | |
 COII | C2-18519 | CCATAACAGGACTAGCAGCATC |  |  |
Gene | 3′ Facing | Sequence | 5′ Facing | Sequence |
---|---|---|---|---|
B. Primers used in cRT-PCR | ||||
 COI | C1-1324 | TACTCGGATTTCCCTGATGC | C1-251 | AACCAATTTCCGAATCCTCCG |
 CytB–ND6 | N6-4962 | TTTGGTTAGTTATTGCCTTT | CB-4128a | GCTCCCCAAAAGGACATTTGTC |
 ND3–ND4–mtMutS | M-8624 | TGATTCGCCAGTTCGGTGCT | M-6780a | TTAAACCTACCCCCGAGTCC |
 ND2–ND5 | N5-14119 | GCTCAGTTTGGAAGTTTGGC | N2-11753a | ACATCGGGAGCCCACATA |
 ND4 | N4-15714 | TTTTGGGCAACTTTCTCC | N4-14532 | CAGAGACCACTCTAACGCTTGTTG |
 COII–ATP8–ATP6 | C2-18161 | GGTTGAAGGTCACTCGTAGGTATC | A6-17048 | GGGTTCGCAATGATTAGTAATGGAATGT |
Gene | Primer | Sequence |
---|---|---|
C. Gene-specific primers used for RT for cRT-PCR analysis | ||
 ATP6 | A6-17092 | TTAGCAGCCAATCGAACACC |
 ND4 | N4-15295 | GCGTCTACCTGTCTGCAAGT |
 mtMutS | M-8826 | CATTTCGGGATGGTAGCTCC |