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Table 2 Primers used in this study

From: Mitochondrial RNA processing in absence of tRNA punctuations in octocorals

Gene

5′ RACE

Sequence

3′ RACE

Sequence

A. Primers used in 5′ and 3′ RACE amplification

 COI

C1-839a

ATCATAGCATAGACCATACC

  

C1-1144

CATAGTGGAAGTGAGCTACTAC

  

 CytB–ND6

CB-4128a

GCTCCCCAAAAGGACATTTGTC

  

 ND3–ND4–mtMutS

N3-5602

CACATTCATAGACCGACACTT

N4L-6071

GCCATTATGGTTAACTATTAC

M-6402

ACGAAGCAACTTGTTCAATGG

M-6363

ATTGAACAAGTTGCYTCGTTACTTG

M-6719

CCGGGTTACTTTGTCCCTGTCCG

M-8714

GCCCTCTCAATATGGCATTG

  

M-6655

CAGCCATGAATGGGCATAG

  

M-8067

GCATTAAGCGGGGCTATTGCGG

 COII

C2-18519

CCATAACAGGACTAGCAGCATC

  

Gene

3′ Facing

Sequence

5′ Facing

Sequence

B. Primers used in cRT-PCR

 COI

C1-1324

TACTCGGATTTCCCTGATGC

C1-251

AACCAATTTCCGAATCCTCCG

 CytB–ND6

N6-4962

TTTGGTTAGTTATTGCCTTT

CB-4128a

GCTCCCCAAAAGGACATTTGTC

 ND3–ND4–mtMutS

M-8624

TGATTCGCCAGTTCGGTGCT

M-6780a

TTAAACCTACCCCCGAGTCC

 ND2–ND5

N5-14119

GCTCAGTTTGGAAGTTTGGC

N2-11753a

ACATCGGGAGCCCACATA

 ND4

N4-15714

TTTTGGGCAACTTTCTCC

N4-14532

CAGAGACCACTCTAACGCTTGTTG

 COII–ATP8–ATP6

C2-18161

GGTTGAAGGTCACTCGTAGGTATC

A6-17048

GGGTTCGCAATGATTAGTAATGGAATGT

Gene

Primer

Sequence

C. Gene-specific primers used for RT for cRT-PCR analysis

 ATP6

A6-17092

TTAGCAGCCAATCGAACACC

 ND4

N4-15295

GCGTCTACCTGTCTGCAAGT

 mtMutS

M-8826

CATTTCGGGATGGTAGCTCC

  1. aIndicates the other primers used for RT during cRT-PCR