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Table 3 Probe sequence for shift assays

From: Early growth response protein 1 regulates promoter activity of α-plasma membrane calcium ATPase 2, a major calcium pump in the brain and auditory system

EGR1 probes Sequence
[−71 to −98] GCC CGA GGG GAG CGG GGG AGG AGA GAG C
Modified [−71 to −98] (Mod) GCC CGA A GG T AG C A G GG T AGG AGA GAG C
Santa Cruz positive control (SC) CGA CGC TGC GTG GGC GGA GCG GGG GCG A
Canonical positive control (Can) GGA TCC AGC GGG GGC GAGCGG GGG CGA
  1. The SC positive control probe was purchased from Santa Cruz Biotechnology. The Can probe was designed utilizing the canonical consensus sequence found on Motifmap (34). Predicted binding sites for EGR1 are underlined in the probe sequence. Bases in the modified probe (Mod) that differ from the [−71 to −98] probe are italicized