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Fig. 3 | BMC Molecular Biology

Fig. 3

From: Early growth response protein 1 regulates promoter activity of α-plasma membrane calcium ATPase 2, a major calcium pump in the brain and auditory system

Fig. 3

Transcription factors of interest and their binding sites in the αAtp2b2 [+101/−2133] promoter. Transcription factors were selected based on a multi-faceted in silico search. a Predicted transcription factor binding sites were found using software (TFBIND and MatInspector) or manually identified using published consensus sequences [17]. Hair cell expression was determined utilizing SHIELD data [27, 39]. SHIELD RNA-Seq data was collected from FACS sorted GFP expressing hair cells, transcripts with reads above zero were considered expressed and denoted in the table with a (+). b Transcription factor expression in OC-1 cells was determined utilizing qPCR and cross-referenced with published microarray data. EGR1, GATA3 and USF1 are expressed [2527, 40]. Data is the average of three technical replicates for three biological replicates, variation is shown as standard error of the mean. c Predicted binding sites for ATOH1, EGR1, GATA3, POU4F3 and USF1 are shown. Note the clustering of predicted binding sites for transcription factors in the CpG island

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