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Table 2 Statistical analysis of mRNA expression of α-SMA and ß-actin in mice kidneys underwent UUO

From: Selective measurement of α smooth muscle actin: why β-actin can not be used as a housekeeping gene when tissue fibrosis occurs

Applied primer Normality test Comparison test (control vs. UUO)
Test P value Test P value
Control UUO
mα-SMASD Kolmogorov–Smirnov 0.2 0.2 Unpaired t test <0.0001
mα-SMAL1 Kolmogorov–Smirnov 0.0115 0.2 Mann–whitney U test 0.0023
mα-SMAL2 Kolmogorov–Smirnov 0.2 0.2 Unpaired t test <0.0001
mα-SMAL3 Kolmogorov–Smirnov 0.2 0.2 Unpaired t test <0.0001
mß-actinSD Kolmogorov–Smirnov 0.1283 0.2 Unpaired t test 0.4187
mß-actinL1 Kolmogorov–Smirnov 0.2 0.2 Unpaired t test 0.0022
mß-actinL2 Kolmogorov–Smirnov 0.2 0.2 Unpaired t test 0.0047
mß-actinL3 Kolmogorov–Smirnov 0.2 0.2 Unpaired t test 0.0021
  1. The mRNA expression of α-SMA and ß-actin was determined by comparison with the expression of 18S ribosomal RNA (RN18S) as internal control from the same samples using the ∆∆Ct method