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Table 1 Primer sequences and parameters

From: Selective measurement of α smooth muscle actin: why β-actin can not be used as a housekeeping gene when tissue fibrosis occurs

Name Species Primer pairs Product length (bp) Ta (°C)
mα-SMASD Mouse F: 5′-CCCCTGAAGAGCATCGGACA-3′ 105 60
R: 5′-TGGCGGGGACATTGAAGGT-3′
mß-actinSD Mouse F: 5′-CCCCTGAGGAGCACCGTGTG-3′ 106 60
R: 5′-ATGGCTGGGGTGTTGAAGGT-3′
mα-SMAL1 Mouse F: 5′-CACTATTGGCAACGAGCGC-3′ 60 48
R: 5′-CCAATGAAGGAAGGCTGGAA-3′
mα-SMAL2 Mouse F: 5′-GTCCCAGACATCAGGGAGTAA-3′ 101 57
R: 5′-TCGGATACTTCAGCGTCAGGA-3′
mα-SMAL3 Mouse F: 5′-GAGGCACCACTGAACCCTAA-3′ 154 54
R: 5′-CATCTCCAGAGTCCAGCACA-3′
mß-actinL1 Mouse F: 5′-GGCTGTATTCCCCTCCATCG-3′ 154 56
R: 5′-CCAGTTGGTAACAATGCCATGT-3′
mß-actinL2 Mouse F: 5′-TGTTACCAACTGGGACGACA-3′ 165 55
R: 5′-GGGGTGTTGAAGGTCTCAAA-3′
mß-actinL3 Mouse F: 5′-AGCCATGTACGTAGCCATCC-3′ 228 57
R: 5′-CTCTCAGCTGTGGTGGTGAA-3′
RN18S Mouse F: 5′-AGCGGTCGGCGTCCCCCAACTTCT-3′ 107 60
R: 5′-GCGCGTGCAGCCCCGGACATCTA-3′
hα-SMASD Human F: 5′-ACTGAGCGTGGCTATTCCTCCGTT-3′ 111 58
R: 5′-GCAGTGGCCATCTCATTTTCA-3′
hß-actinSD Human F: 5′-ACCGAGCGTGGCTACAGCTTCACC-3′ 114 53
R: 5′-AGCACCCGTGGCCATCTCTTTCTCG-3′
rα-SMASD Rat F: 5′-GAGCGTGGCTATTCCTTCGTG-3′ 106 54
R: 5′-CAGTGGCCATCTCATTTTCAAAGT-3′
rß-actinSD Rat F: 5′-ACCGAGCATGGCTACAGCGTCACC-3′ 106 54
R: 5′-GTGGCCATCTCTTGCTCGGAGTCT-3′
  1. Nucleotide sequences of forward (F) and reverse (R) primers, product lengths and specific optimal annealing temperatures (Ta) applied for the real-time PCR detection
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