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Table 1 Primer sequences and parameters

From: Selective measurement of α smooth muscle actin: why β-actin can not be used as a housekeeping gene when tissue fibrosis occurs

Name

Species

Primer pairs

Product length (bp)

Ta (°C)

mα-SMASD

Mouse

F:

5′-CCCCTGAAGAGCATCGGACA-3′

105

60

R:

5′-TGGCGGGGACATTGAAGGT-3′

mß-actinSD

Mouse

F:

5′-CCCCTGAGGAGCACCGTGTG-3′

106

60

R:

5′-ATGGCTGGGGTGTTGAAGGT-3′

mα-SMAL1

Mouse

F:

5′-CACTATTGGCAACGAGCGC-3′

60

48

R:

5′-CCAATGAAGGAAGGCTGGAA-3′

mα-SMAL2

Mouse

F:

5′-GTCCCAGACATCAGGGAGTAA-3′

101

57

R:

5′-TCGGATACTTCAGCGTCAGGA-3′

mα-SMAL3

Mouse

F:

5′-GAGGCACCACTGAACCCTAA-3′

154

54

R:

5′-CATCTCCAGAGTCCAGCACA-3′

mß-actinL1

Mouse

F:

5′-GGCTGTATTCCCCTCCATCG-3′

154

56

R:

5′-CCAGTTGGTAACAATGCCATGT-3′

mß-actinL2

Mouse

F:

5′-TGTTACCAACTGGGACGACA-3′

165

55

R:

5′-GGGGTGTTGAAGGTCTCAAA-3′

mß-actinL3

Mouse

F:

5′-AGCCATGTACGTAGCCATCC-3′

228

57

R:

5′-CTCTCAGCTGTGGTGGTGAA-3′

RN18S

Mouse

F:

5′-AGCGGTCGGCGTCCCCCAACTTCT-3′

107

60

R:

5′-GCGCGTGCAGCCCCGGACATCTA-3′

hα-SMASD

Human

F:

5′-ACTGAGCGTGGCTATTCCTCCGTT-3′

111

58

R:

5′-GCAGTGGCCATCTCATTTTCA-3′

hß-actinSD

Human

F:

5′-ACCGAGCGTGGCTACAGCTTCACC-3′

114

53

R:

5′-AGCACCCGTGGCCATCTCTTTCTCG-3′

rα-SMASD

Rat

F:

5′-GAGCGTGGCTATTCCTTCGTG-3′

106

54

R:

5′-CAGTGGCCATCTCATTTTCAAAGT-3′

rß-actinSD

Rat

F:

5′-ACCGAGCATGGCTACAGCGTCACC-3′

106

54

R:

5′-GTGGCCATCTCTTGCTCGGAGTCT-3′

  1. Nucleotide sequences of forward (F) and reverse (R) primers, product lengths and specific optimal annealing temperatures (Ta) applied for the real-time PCR detection