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Table 1 Primer sequences and parameters
| Name | Species | Primer pairs | Product length (bp) | Ta (°C) | |
|---|---|---|---|---|---|
| mα-SMASD | Mouse | F: | 5′-CCCCTGAAGAGCATCGGACA-3′ | 105 | 60 |
| R: | 5′-TGGCGGGGACATTGAAGGT-3′ | ||||
| mß-actinSD | Mouse | F: | 5′-CCCCTGAGGAGCACCGTGTG-3′ | 106 | 60 |
| R: | 5′-ATGGCTGGGGTGTTGAAGGT-3′ | ||||
| mα-SMAL1 | Mouse | F: | 5′-CACTATTGGCAACGAGCGC-3′ | 60 | 48 |
| R: | 5′-CCAATGAAGGAAGGCTGGAA-3′ | ||||
| mα-SMAL2 | Mouse | F: | 5′-GTCCCAGACATCAGGGAGTAA-3′ | 101 | 57 |
| R: | 5′-TCGGATACTTCAGCGTCAGGA-3′ | ||||
| mα-SMAL3 | Mouse | F: | 5′-GAGGCACCACTGAACCCTAA-3′ | 154 | 54 |
| R: | 5′-CATCTCCAGAGTCCAGCACA-3′ | ||||
| mß-actinL1 | Mouse | F: | 5′-GGCTGTATTCCCCTCCATCG-3′ | 154 | 56 |
| R: | 5′-CCAGTTGGTAACAATGCCATGT-3′ | ||||
| mß-actinL2 | Mouse | F: | 5′-TGTTACCAACTGGGACGACA-3′ | 165 | 55 |
| R: | 5′-GGGGTGTTGAAGGTCTCAAA-3′ | ||||
| mß-actinL3 | Mouse | F: | 5′-AGCCATGTACGTAGCCATCC-3′ | 228 | 57 |
| R: | 5′-CTCTCAGCTGTGGTGGTGAA-3′ | ||||
| RN18S | Mouse | F: | 5′-AGCGGTCGGCGTCCCCCAACTTCT-3′ | 107 | 60 |
| R: | 5′-GCGCGTGCAGCCCCGGACATCTA-3′ | ||||
| hα-SMASD | Human | F: | 5′-ACTGAGCGTGGCTATTCCTCCGTT-3′ | 111 | 58 |
| R: | 5′-GCAGTGGCCATCTCATTTTCA-3′ | ||||
| hß-actinSD | Human | F: | 5′-ACCGAGCGTGGCTACAGCTTCACC-3′ | 114 | 53 |
| R: | 5′-AGCACCCGTGGCCATCTCTTTCTCG-3′ | ||||
| rα-SMASD | Rat | F: | 5′-GAGCGTGGCTATTCCTTCGTG-3′ | 106 | 54 |
| R: | 5′-CAGTGGCCATCTCATTTTCAAAGT-3′ | ||||
| rß-actinSD | Rat | F: | 5′-ACCGAGCATGGCTACAGCGTCACC-3′ | 106 | 54 |
| R: | 5′-GTGGCCATCTCTTGCTCGGAGTCT-3′ | ||||
