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Fig. 8 | BMC Molecular Biology

Fig. 8

From: Selective measurement of α smooth muscle actin: why β-actin can not be used as a housekeeping gene when tissue fibrosis occurs

Fig. 8

Representative plots of real-time PCRs using literary primer pairs specific for mouse ß-actin. Real-time PCRs using mß-actinL1, mß-actinL2 or mß-actinL3 primer pairs and mß-actinT DNA templates resulted in products (CtL1 = 19.73, CtL2 = 27.72, CtL3 = 18.65) with single melting peaks at 85.6, 86.3, or 87.4 °C and in discrete bands in the agarose gel at 60, 101 or 154 bp, respectively (ac). Real-time PCRs using mß-actinL1, mß-actinL2 or mß-actinL3 literary primer pairs amplified the non-specific mα-SMAT DNA templates also (CtL1 = 31.29, CtL2 = 21.08, CtL3 = 28.43), resulted in melting peaks at 85.6, 84.2 or 87.4 °C and indiscrete bands at 60, 101 or 154 bp, respectively (ac)

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