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Fig. 7 | BMC Molecular Biology

Fig. 7

From: Selective measurement of α smooth muscle actin: why β-actin can not be used as a housekeeping gene when tissue fibrosis occurs

Fig. 7

Representative plots of real-time PCRs using literary primer pairs specific for mouse α-SMA. Real-time PCRs using mα-SMAL1, mα-SMAL2 or mα-SMAL3 primer pairs and mα-SMAT DNA templates resulted in products (CtL1 = 28.09, CtL2 = 17.19, CtL3 = 16.79) with single melting peaks at 82, 81.9, or 83.3 °C and in discrete bands in the agarose gel at 60, 101 or 154 bp, respectively (ac). Real-time PCRs using mα-SMAL1, mα-SMAL2 or mα-SMAL3 literary primer pairs amplified the non-specific mß-actinT DNA templates also (CtL1 = 31.09, CtL2 = 34.62, CtL3 = 32.96), resulted in melting peaks at 81, 81.9, or 83.3 °C and in discrete bands at 60, 101 or 154 bp, respectively (ac)

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