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Fig. 2 | BMC Molecular Biology

Fig. 2

From: Selective measurement of α smooth muscle actin: why β-actin can not be used as a housekeeping gene when tissue fibrosis occurs

Fig. 2

Representative plots of mouse α-SMA specific real-time PCRs. PCRs using mα-SMASD primer pair amplified mα-SMAT artificial DNA template (Ct = 25.64) (a), but did not amplify the mß-actinT, mγ-cyto-actinT and mγ-smooth-actinT DNA fragments (bd). Our mouse α-SMA specific PCR resulted in a product with single melting peak at 83.4 °C (e) and in one discrete band at 105 bp in agarose gel after electrophoresis (f)

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