Fig. 11
Representative plots of human and rat α-SMA and ß-actin specific real-time PCRs. PCRs using hα-SMASD or rα-SMASD specific primer pairs amplified the hα-SMAT (Ct = 32) or rα-SMAT (Ct = 28.21) DNA template (a, b respectively), but did not amplify the hß-actinT or rß-actinT template (a, b respectively). Our human or rat α-SMA specific PCRs resulted in products with melting peaks at 81.7 or 82.8 °C, and in discrete bands in agarose gel at 111 or 106 bp, respectively (a, b). Similarly, our hß-actinSD or rß-actinSD primer pair amplified the hß-actinT (Ct = 27.42) or rß-actinT (Ct = 25.58) DNA template (c, d respectively), but did not amplify the hα-SMAT or rα-SMAT template (c, d respectively). Our human or rat ß-actin specific PCRs resulted in products with melting peaks at 86 or 83.4 °C, and in discrete bands in agarose gel at 114 or 107 bp, respectively (c, d)