Fig. 4

Active DNA demethylation at SPI1 regulatory regions. a Quantification of the concentration of mitomycin-C for cell growth arrest at the G₁ phase. b–d Quantification of DNA methylation at the −17-kb URE with 50 µg/ml mitomycin-C treatment. b The DNA methylation pattern was measured at the −17-kb URE of MCS- and RUNX1-overexpressing HEK-293T cells. c The percentage of methylation in MCS- and RUNX1-overexpressing HEK-293T cells. d The methylation difference at each individual CpG site between MCS- and RUNX1-overexpressing HEK-293T cells. All of the sites were significantly demethylated (***p < 0.0001). e–g Quantification of DNA methylation at the proximal promoter upon 50 µg/ml mitomycin-C treatment. e The DNA methylation pattern was measured at the proximal promoter region of MCS- and RUNX1-overexpressing HEK-293T cells. f Percentage of methylation in MCS- and RUNX1-overexpressing HEK-293T cells. g The methylation difference at each individual CpG site in MCS- and RUNX1-overexpressing HEK-293T cells. All of the sites were significantly demethylated (***p < 0.0001). h mRNA expression level of genes involved in DNA methylation and demethylation in MCS- and RUNX1-overexpressing HEK-293T cells (Expression value quality control processed signals from microarray data. Error bar standard deviation of biological triplicates)