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Fig. 3 | BMC Molecular Biology

Fig. 3

From: RUNX1 induces DNA replication independent active DNA demethylation at SPI1 regulatory regions

Fig. 3

DNA methylation status analysis of SPI1 proximal promoter in RUNX1-overexpressing HEK-293T cells: a Target sequence of the human SPI1 proximal promoter region. The number represents the CpG sites and the SPI1 binding site is also shown. b Methylation pattern diagrams of wild-type, MCS, and RUNX1-overexpressing HEK-293T cells at the SPI1 proximal promoter region. Each row of circles represents the result of a single amplicon and each column represents a single CpG site. Black circles denote methylated CpGs and open circles represent unmethylated ones. The CpG numbers shown correspond to the target nucleotide sequence, shown in a. c Quantification of percent CpG methylation at the proximal promoter region in wild-type, MCS, and RUNX1-overexpressing HEK-293T cells. The p value calculated from the comparison between MCS and RUNX1-overexpressing HEK-293T cells was significant (***p < 0.0001). d Comparison of methylation status graph at each individual CpG site between MCS and RUNX1-overexpressing HEK-293T cells. The significantly demethylated CpG sites are marked with asterisks, ***p < 0.005, **p < 0.01, *p < 0.05

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