Table 1 Name, primer sequences, and product size of candidate reference genes
Symbol | Gene name | Primer sequences (forward/reverse) | Product length (bp) |
|---|---|---|---|
18S | 18S ribosomal RNA | CGGCTACCACATCCAAGGAA/GCTGGAATTACCGCGGCT | 186 |
GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | GACAGTCAGCCGCATCTTCT/TTAAAAGCAGCCCTGGTGAC | 120 |
U6 | U6 snRNA | AACGCTTCACGAATTTGCGT/CTCGCTTCGGCAGCACA | 109 |
ALAS1 | 5′-Aminolevulinate synthase 1 | GGCAGCACAGATGAATCAGA/CCTCCATCGGTTTTCACACT | 150 |
ACTB | Actin, beta | AGAAAATCTGGCACCACACC/TAGCACAGCCTGGATAGCAA | 173 |
TFRC | Transferrin receptor | GTCGCTGGTCAGTTCGTGATT/AGCAGTTGGCTGTTGTACCTCTC | 80 |
PPIA | Peptidylprolyl isomerase A | AGACAAGGTCCCAAAGAC/ACCACCCTGACACATAAA | 118 |
RPLP0 | Ribosomal protein lateral stalk subunit P0 | CCATTCTATCATCAACGGGTACAA/TCAGCAAGTGGGAAGGTGTAAT | 75 |
PBGD | Hydroxymethylbilane synthase | AGTGTGGTGGGAACCAGC/CAGGATGATGGCACTGAACTC | 144 |
GUSB | Glucuronidase beta | AGCCAGTTCCTCATCAATGG/GGTAGTGGCTGGTACGGAAA | 160 |
B2M | Beta-2-microglobulin | AGCGTACTCCAAAGATTCAGGTT/ATGATGCTGCTTACATGTCTCGAT | 206 |
HPRT1 | Hypoxanthine phosphoribosyl transferase 1 | GACCAGTCAACAGGGGACAT/CCTGACCAAGGAAAGCAAAG | 132 |
RPL29 | Ribosomal protein L29 | GGCGTTGTTGACCCTATTTC/GTGTGTGGTGTGGTTCTTGG | 120 |
PUM1 | Pumilio RNA binding family member 1 | CAGGCTGCCTACCAACTCAT/GTTCCCGAACCATCTCATTC | 211 |
TBP | TATA-box binding protein | TGCACAGGAGCCAAGAGTGAA/CACATCACAGCTCCCCACCA | 132 |