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Fig. 2 | BMC Molecular Biology

Fig. 2

From: Identification of suitable reference genes for real-time quantitative PCR analysis of hydrogen peroxide-treated human umbilical vein endothelial cells

Fig. 2

Expression stability values of reference genes analyzed by geNorm software. a Average expression stability measures (M) of reference genes. The x-axis from left to right indicates the ranking of the genes according to their stability; higher M values indicate lower stabilities. b Determination of the suitable number of reference genes required for normalizing. The software calculates the normalization factor from at least two genes, and the V value defines the pair-wise variation between two sequential normalization factors

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