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Fig. 1 | BMC Molecular Biology

Fig. 1

From: Properties of STAT1 and IRF1 enhancers and the influence of SNPs

Fig. 1

IFNγ target subclasses. a Human chromosome ideograms, drawn using NCBI Map Viewer, show locations of the 11 studied chromosomal segments (red arrows). b The 382 genes within these regions were classified as “IFNγ stimulated genes” (ISGs), “IFNγ-repressed genes” (IRGs) or “other genes” according to our expression array data and on prior studies (see “Methods” and “Results” sections for details). Known ISGs (in this and other studies) were either induced (indISGs) or resistant (resISGs) in HeLa cells. Genes were further subcategorized according to their robustness (strong/weak) and timing (early/late) of induction/repression (e.g. early strong induced ISGs: es-indISGs). Other genes were termed “not-linked to IFNγ”, or “potential ISGs” (potISGs) if they were either ISG paralogs or exhibited IFNγ-induced TF binding at their promoter. Italics indicate the number of CIITA targets in each class. c To validate array data, mRNA was isolated from HeLa cells left untreated or exposed to IFNγ for 6 h. RT-PCR was performed on 24 induced (IndISGs) or resistant (ResISGs) ISGs, selected based on array data 6 h after IFNγ treatment. Pie diagram shows the percentage of validated genes

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