Fig. 4
Confirmation of the accuracy of MSD-AFLP analysis by MSRE-PCR. a Relative methylation levels of 11 CpG sites in Fig. 3 determined by MSRE-PCR analysis using locus-specific primers. b Relative fluorescence intensity obtained from MSD-AFLP data. c Scatter plot of relative methylation level determined using MSRE-PCR analysis and relative fluorescence intensity determined using MSD-AFLP analysis. The dashed line indicates y = x whereas the solid line indicates the line of best fit. A high coefficient of correlation was determined (R2 = 0.9787)