Table 1 Oligonucleotides used in this study
From: Transcriptional regulation of human sperm-associated antigen 16 gene by S-SOX5
Primer name | Sequence (5′–3′) | Application |
|---|---|---|
SPAG16L-promoterF1 | GGTACCGGTCAAAGCGAAAGAAAACC | Forward primer for transcription fusion to luc |
SPAG16L-promoterR2 | CTCGAGGAACAGCGAAGACGCTACCC | Reverse primer for transcription fusion to luc |
Human S-SOX5 forward | GTGCCATAGGAGCTGTGCATG | S-SOX5 expression |
Human S-SOX5 reverse | GTTGGTCCTTCATTTGCCGAGC | |
SOX5 RNAi (225) sense | AAAAATGATGCTGTCACCAAGGCAA | SOX5 RNAi construction |
SOX5 RNAi (225) anti-sense | AAAGTTGCCTTGGTGACAGCATCAT | |
SOX5 RNAi (1109) sense | AAAAGATTATGGGAGTGACAGTGAA | SOX5 RNAi construction |
SOX5 RNAi (1109) anti-sense | AAAGTTCACTGTCACTCCCATAATC | |
SPAG16L RT-PCRF | TTCAGACTGCTGCTTCCATC | Real-time PCR analysis of SPAG16L |
SPAG16L RT-PCRR | TCGCCTGTACATAGATCCCA | |
GAPDH RT-PCRF | GGAGGTGAAGGTCGGAGTC | Real-time PCR analysis of GAPDH |
GAPDH RT-PCRR | GAAGATG GTGATGGGATTTC | |
SPAG16L mutation 1F | TGCAATGCAAGCCAACCACCTACTGTATCTTGTCC | Mutation of SOX5 binding site (P-I site) at SPAG16L promoter |
SPAG16L mutation 1R | GGACAAGATACAGTAGGTGGTTGGCTTGCATTGCA | |
SPAG16L mutation 2F | GTTAACTAGGCAACACTACCGCCACGGTAACTGGG | Mutation of SOX5 binding site (P-II site) at SPAG16L promoter |
SPAG16L mutation 2R | CCCAGTTACCGTGGCGGTAGTGTTGCCTAGTTAAC | |
SPAG16L ChIP-aF | CTTGGGTGACTTCCAATTTTG | ChIP assays for SPAG16L site a |
SPAG16L ChIP-aR | GTATCATTAACTACACTCCTC | |
SPAG16L ChIP-bF | CCTACTTGAGGAGGAGAGTGGGA | ChIP assays for SPAG16L site b |
SPAG16L ChIP-bR | TATCGCGTATGTATCAGAAGC | |
SPAG16L ChIP-cF | GGAAGATCCTCTCAGCAATAAGAC | ChIP assays for SPAG16L site c |
SPAG16L ChIP-cR | GAAGAACTATGGTGTTCAGC |