Fig. 3
From: Transcriptional regulation of human sperm-associated antigen 16 gene by S-SOX5
Knockdown of S-SOX5 in BEAS-2B cells by RNAi results in decrease of SPAG16L mRNA. a Western blot analysis of S-SOX5 proteins in BEAS-2B cells stably expressing two RNAi constructs. b After RNAi treatment, the levels of S-SOX5 in BEAS-2B cells were estimated by analysing intensity of protein bands using ImageJ software. c Analysis of SPAG16L mRNA expression by real-time PCR in BEAS-2B cells transfected with two SOX5 RNAi constructs. Silencing of S-SOX5 by RNAi reduced expression of SPAG16L. Values indicate mean ± S.E. (error bars) of the relative mRNA level (n = 3). *p < 0.05 (Student’s t test) compared to the control