Fig. 5

Interaction between Ago-miR-656a-3p and CYP6J1 using a dual fluorescent reporter system. a Predicted target sites of Ago-miR-656a-3p in the 3′ UTR of CYP6J1. b Luciferase reporter assays performed by co-transfection of Ago-miR-656a-3p agomir with a luciferase reporter gene linked to the 3′ UTR of CYP6J1. Firefly luciferase activity was normalized to Renilla luciferase activity and then normalized to the activity of the control group. The mathematical operators of “+”and “−” mean add and subtract. Different letters on the bars of the histogram indicate significant differences based on ANOVA followed by Tukey’s HSD multiple comparison test (P < 0.05)