Binding affinity of five PBPs to Ostrinia sex pheromones

BMC Molecular Biology

Table 3 Competitive ligand binding assay results from four Ostrinia furnacalis PBP3 (OfurPBP3) mutants generated by site-directed mutagenesis of Ile113 to Asn113 (M1: I113N), Phe12 to Ser12 (M2: F12S), Ile52 to Asn52 (M3: I52N), and Ile94 to Arg94 (M4: I94R)

	Ostrinia nubilalis female sex pheromones				Ostrinia furnacalis female sex pheromones				Other lepidopteran female sex pheromones
	Z11-14:OAc		E11-14:OAc		Z12-14:OAc		E12-14:OAc		Z9-14:OH		E11-14:OH
	IC₅₀	Ki	IC₅₀	Ki	IC₅₀	Ki	IC₅₀	Ki	IC₅₀	Ki	IC₅₀	Ki
OfurPBP3	3.27 ± 0.24	2.44 ± 0.10c	2.64 ± 0.15	1.97 ± 0.07b	3.83 ± 0.06	2.85 ± 0.02b	3.81 ± 0.02	2.84 ± 0.01b	–	–	–	–
M1 I113 N	27.81 ± 9.01	20.11 ± 3.76a	11.00 ± 3.86	5.57 ± 1.61ab	6.78 ± 2.27	4.91 ± 0.95ab	8.03 ± 1.77	11.60 ± 3.81ab	6.14 ± 1.18	4.42 ± 0.85	10.38 ± 1.20	8.08 ± 0.33
M2 F12S	10.58 ± 1.24	6.53 ± 0.57bc	8.17 ± 0.28	6.53 ± 0.13ab	6.09 ± 0.57	4.89 ± 0.27ab	7.47 ± 0.87	6.02 ± 0.40ab	9.52 ± 1.28	7.69 ± 1.02	12.35 ± 2.58	9.87 ± 2.06
M3 I52 N	13.29 ± 2.16	12.96 ± 1.04ab	14.97 ± 0.10	12.48 ± 0.05a	11.92 ± 4.37	10.65 ± 2.10a	18.77 ± 3.30	13.03 ± 1.59ab	18.33 ± 1.31	16.35 ± 1.09	15.45 ± 2.50	12.87 ± 2.09
M4 I94R	9.83 ± 0.76	8.71 ± 0.39bc	12.65 ± 2.11	13.78 ± 2.13a	10.59 ± 1.71	9.50 ± 0.88ab	17.40 ± 3.49	15.42 ± 1.79a	12.01 ± 2.24	10.81 ± 1.99	11.09 ± 2.50	10.47 ± 2.22

Mutant and wildtype OfurPBP proteins were assayed against six female sex pheromones found among species in the genus Ostrinia. All assays performed at pH = 7.4. Values reported as μM concentrations of each pheromone component
IC₅₀ is the ligand concentration displacing 50% of the fluorescent reporter; K_i is the binding constant calculated from the equation K_i = [IC₅₀]/(1 + [1-NPN]/K_1-NPN)
Mean ± SE, date in the same column followed by the same letters were not significantly different (P ≥ 0.05) according to LSD test. Dashes indicate undetectable interations

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