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Table 3 Competitive ligand binding assay results from four Ostrinia furnacalis PBP3 (OfurPBP3) mutants generated by site-directed mutagenesis of Ile113 to Asn113 (M1: I113N), Phe12 to Ser12 (M2: F12S), Ile52 to Asn52 (M3: I52N), and Ile94 to Arg94 (M4: I94R)

From: Binding affinity of five PBPs to Ostrinia sex pheromones

  Ostrinia nubilalis female sex pheromones Ostrinia furnacalis female sex pheromones Other lepidopteran female sex pheromones
  Z11-14:OAc E11-14:OAc Z12-14:OAc E12-14:OAc Z9-14:OH E11-14:OH
  IC50 Ki IC50 Ki IC50 Ki IC50 Ki IC50 Ki IC50 Ki
OfurPBP3 3.27 ± 0.24 2.44 ± 0.10c 2.64 ± 0.15 1.97 ± 0.07b 3.83 ± 0.06 2.85 ± 0.02b 3.81 ± 0.02 2.84 ± 0.01b
M1 I113 N 27.81 ± 9.01 20.11 ± 3.76a 11.00 ± 3.86 5.57 ± 1.61ab 6.78 ± 2.27 4.91 ± 0.95ab 8.03 ± 1.77 11.60 ± 3.81ab 6.14 ± 1.18 4.42 ± 0.85 10.38 ± 1.20 8.08 ± 0.33
M2 F12S 10.58 ± 1.24 6.53 ± 0.57bc 8.17 ± 0.28 6.53 ± 0.13ab 6.09 ± 0.57 4.89 ± 0.27ab 7.47 ± 0.87 6.02 ± 0.40ab 9.52 ± 1.28 7.69 ± 1.02 12.35 ± 2.58 9.87 ± 2.06
M3 I52 N 13.29 ± 2.16 12.96 ± 1.04ab 14.97 ± 0.10 12.48 ± 0.05a 11.92 ± 4.37 10.65 ± 2.10a 18.77 ± 3.30 13.03 ± 1.59ab 18.33 ± 1.31 16.35 ± 1.09 15.45 ± 2.50 12.87 ± 2.09
M4 I94R 9.83 ± 0.76 8.71 ± 0.39bc 12.65 ± 2.11 13.78 ± 2.13a 10.59 ± 1.71 9.50 ± 0.88ab 17.40 ± 3.49 15.42 ± 1.79a 12.01 ± 2.24 10.81 ± 1.99 11.09 ± 2.50 10.47 ± 2.22
  1. Mutant and wildtype OfurPBP proteins were assayed against six female sex pheromones found among species in the genus Ostrinia. All assays performed at pH = 7.4. Values reported as μM concentrations of each pheromone component
  2. IC50 is the ligand concentration displacing 50% of the fluorescent reporter; Ki is the binding constant calculated from the equation Ki = [IC50]/(1 + [1-NPN]/K1-NPN)
  3. Mean ± SE, date in the same column followed by the same letters were not significantly different (P ≥ 0.05) according to LSD test. Dashes indicate undetectable interations