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Table 1 Competitive ligand binding assay results among five recombinant native Ostrinia furnacalis PBP orthologs (OfurPBP1 to 5) tested against six female sex pheromones found among species in the genus Ostrinia

From: Binding affinity of five PBPs to Ostrinia sex pheromones

 

Ostrinia nubilalis female sex pheromones

Ostrinia furnacalis female sex pheromones

Other lepidopteran female sex pheromones

Z11-14:OAc

E11-14:OAc

Z12-14:OAc

E12-14:OAc

Z9-14:OH

E11-14:OH

IC50

Ki

IC50

Ki

IC50

Ki

IC50

Ki

IC50

Ki

IC50

Ki

OfurPBP1

4.69 ± 0.14

4.08 ± 0.12a

5.30 ± 0.02

4.61 ± 0.02a

4.88 ± 0.94

4.24 ± 0.82a

5.77 ± 0.16

5.01 ± 0.14a

–

–

–

–

OfurPBP2

3.49 ± 0.06

2.95 ± 0.05b

3.28 ± 0.01

2.77 ± 0.01b

4.96 ± 0.23

4.19 ± 0.09b

4.88 ± 0.10

4.12 ± 0.08b

–

–

16.43 ± 0.18

13.88 ± 0.15

OfurPBP3

3.27 ± 0.24

2.44 ± 0.18c

2.64 ± 0.15

1.97 ± 0.11c

3.83 ± 0.06

2.86 ± 0.04c

3.81 ± 0.02

2.85 ± 0.01c

–

–

–

–

OfurPBP4

1.18 ± 0.05

1.09 ± 0.05d

2.31 ± 0.57

2.13 ± 0.53c

9.97 ± 0.20

9.19 ± 0.18c

–

–

–

–

–

–

OfurPBP5

3.64 ± 0.28

2.18 ± 0.17e

1.90 ± 0.07

1.14 ± 0.04d

11.06 ± 1.13

6.64 ± 0.66d

11.34 ± 0.81

6.80 ± 0.47d

–

–

–

–

  1. All assays performed at pH = 7.4. Values reported as μM concentrations of each pheromone component
  2. IC50 is the ligand concentration displacing 50% of the fluorescent reporter; Ki is the binding constant calculated from the equation Ki = [IC50]/(1 + [1-NPN]/K1-NPN)
  3. Mean ± SE, data in the same column followed by the same letters were not significantly different (P ≥ 0.05) according to LSD test. Dashes indicate undetectable interations