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Table 1 Competitive ligand binding assay results among five recombinant native Ostrinia furnacalis PBP orthologs (OfurPBP1 to 5) tested against six female sex pheromones found among species in the genus Ostrinia

From: Binding affinity of five PBPs to Ostrinia sex pheromones

  Ostrinia nubilalis female sex pheromones Ostrinia furnacalis female sex pheromones Other lepidopteran female sex pheromones
Z11-14:OAc E11-14:OAc Z12-14:OAc E12-14:OAc Z9-14:OH E11-14:OH
IC50 Ki IC50 Ki IC50 Ki IC50 Ki IC50 Ki IC50 Ki
OfurPBP1 4.69 ± 0.14 4.08 ± 0.12a 5.30 ± 0.02 4.61 ± 0.02a 4.88 ± 0.94 4.24 ± 0.82a 5.77 ± 0.16 5.01 ± 0.14a
OfurPBP2 3.49 ± 0.06 2.95 ± 0.05b 3.28 ± 0.01 2.77 ± 0.01b 4.96 ± 0.23 4.19 ± 0.09b 4.88 ± 0.10 4.12 ± 0.08b 16.43 ± 0.18 13.88 ± 0.15
OfurPBP3 3.27 ± 0.24 2.44 ± 0.18c 2.64 ± 0.15 1.97 ± 0.11c 3.83 ± 0.06 2.86 ± 0.04c 3.81 ± 0.02 2.85 ± 0.01c
OfurPBP4 1.18 ± 0.05 1.09 ± 0.05d 2.31 ± 0.57 2.13 ± 0.53c 9.97 ± 0.20 9.19 ± 0.18c
OfurPBP5 3.64 ± 0.28 2.18 ± 0.17e 1.90 ± 0.07 1.14 ± 0.04d 11.06 ± 1.13 6.64 ± 0.66d 11.34 ± 0.81 6.80 ± 0.47d
  1. All assays performed at pH = 7.4. Values reported as μM concentrations of each pheromone component
  2. IC50 is the ligand concentration displacing 50% of the fluorescent reporter; Ki is the binding constant calculated from the equation Ki = [IC50]/(1 + [1-NPN]/K1-NPN)
  3. Mean ± SE, data in the same column followed by the same letters were not significantly different (P ≥ 0.05) according to LSD test. Dashes indicate undetectable interations