Fig. 3

hSSB1 associates with proteins comprising numerous chromatin-remodelling complexes. HeLa whole cell lysates were prepared and 500 μg incubated for 2 h at 4 °C with protein G dynabeads bound to a hSSB1 antibody, or a sheep isotype control IgG. Beads were washed five times and protein eluted by heating to 80 °C in 3× SDS loading buffer for 5 min. Eluent was immunoblotted with antibodies against BAZ1B, SMARCA5, MTA2, RBBP4 and hSSB1 as indicated. 15 μg of whole cell lysate (input) was immunoblotted with antibodies against hSSB1 and actin (loading control)