Fig. 5
From: 3C-digital PCR for quantification of chromatin interactions
Comparison of 3C-dPCR with 3C-qPCR. a Copy number of ligation products at each selected restriction site detected by 3C-dPCR. Relative strong interaction signals were found with the EcoRI fragments covering primer T6, T7 and T11. The y-axis displays the ligation copy numbers at each EcoRI site per 1000 genome. The x-axis is the genomic position of each EcoRI site. The error bars represent 95% CIs. b 3C qPCR-based interaction signals between anchor primers and test primers. Higher interaction signals were detected with the EcoRI fragments covering primer T6, T7 and T11. The y-axis displays normalized 3C-qPCR values. The x-axis is the genomic position of each EcoRI site. Error bars represent standard deviation of triplicate qPCR results. The TaqMan assay at T3, T4 T8, T9, T12 fragments are beyond detection limitation of qPCR (>45Ct). Quantification values may not reflect fragment ligation frequency