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Fig. 4 | BMC Molecular Biology

Fig. 4

From: 3C-digital PCR for quantification of chromatin interactions

Fig. 4

Interactions between 2L and the cluster of EcoRI fragments on CAPG gene locus at 2p11.2. a Anchor primer 2L, TaqMan probe on prostate cancer SNP risk region and eleven test primers (from T1 to T12) around the CAPG gene locus are selected for the detection of the long-range interactions. Small vertical bars in black represent EcoRI digestion sites. Black and grey arrows show the anchor primer and test primers, respectively. The TaqMan probe is depicted as grey bar. b The copy number of ligation products at each selected restriction site. Relative strong interaction signals were found with the EcoRI fragments covering primer T6, T7 and T11. The y-axis displays the ligation copy numbers at each EcoRI site per 1000 genome. The x-axis is the genomic position of each EcoRI site. Error bars represent standard deviation of triplicate dPCR results. A adjacent ligation primer, S self-ligation primer, U undigested control primer, P TaqMan probe. The error bars represent 95% CIs

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