Fig. 3
From: 3C-digital PCR for quantification of chromatin interactions
Interactions between prostate cancer risk region 1 and MYC gene locus at 8q24. a The anchor primer 8L, TaqMan probe; five target test primers (MYC1–MYC5) and three control test primers (9, 85, and 92 kb) are designed for the detection of the cis-acting interactions. Small vertical bars in black represent EcoRI digestion sites. Black and grey arrows show the anchor primer and test primers, respectively. The TaqMan probe is depicted as grey bar. b The copy number of ligation products at each selected restriction site. The highest interaction is at MYC-3 fragment, which contains the MYC promoter region. The y-axis displays the ligation copy numbers at each EcoRI site per 1000 genome. The x-axis is the genomic position of each EcoRI site. The error bars represent 95% CIs