Table 1 Yeast strains used in this study
From: RNA polymerase II depletion promotes transcription of alternative mRNA species
Strain | Original name, genotype (annotation) | Notes | References |
|---|---|---|---|
MVY150 | W303-1B, MATα ade2-1 trp1-1 can1-100 leu2-3, 112 his3-11,15 ura3-1 |  | [7] |
MVY451 | SYY9, MATa ade2-1 his3-11, 15 leu2-3, 112 trp1-1 ura3-1 can1-100 rpb1-1 (derived from W303) | Â | [27] |
MVY845 | NOY408-1a, MATα rpa135::LEU2 ade2-1 ura3-1 trp1-1 leu2-3,112 his3-11 can1-100 carrying pNOY102 (GAL7-35S rDNA URA3) |  | [28] |
MVY851 | MATα rpa135::LEU2 ade2-1 ura3-1 trp1-1 leu2-3,112 his3-11 can1-100 carrying pNOY102 (GAL7-35S rDNA URA3) rpb1-1 |  | This study |
MVY858 | HHYy168, MATα tor1-1 fpr1::NAT RPL13A-2xFKBP12::TRP1 |  | [11] |
MVY859 | HHY170, MATα tor1-1 fpr1::NAT RPL13A-2xFKBP12::TRP1 RPO21-FRB::KANMX6 |  | [11] |
MVY860 | MVY858 with RPA190-FRB::KANMX6 | Â | This study |
MVY862 | MVY858 with RPC160-FRB::KANMX6 | Â | This study |
MVY868 | MVY858 with TBP1-FRB::KANMX6 | Â | This study |
MVY872 | MVY859 with RPA190-FRB-HIS3MX | Â | This study |
MVY874 | MVY859 with RPC160-FRB-HIS3MX | Â | This study |
MVY879 | MVY872 with RPC160-FRB-LEU2 | Â | This study |
CY1940 | MVY858 with RPA190 FLAG | Pol I AA | This study |
CY2032 | MVY859 with RPA190 FLAG | Pol I AA Pol II AA | This study |