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Table 1 Yeast strains used in this study

From: RNA polymerase II depletion promotes transcription of alternative mRNA species

Strain Original name, genotype (annotation) Notes References
MVY150 W303-1B, MATα ade2-1 trp1-1 can1-100 leu2-3, 112 his3-11,15 ura3-1   [7]
MVY451 SYY9, MATa ade2-1 his3-11, 15 leu2-3, 112 trp1-1 ura3-1 can1-100 rpb1-1 (derived from W303)   [27]
MVY845 NOY408-1a, MATα rpa135::LEU2 ade2-1 ura3-1 trp1-1 leu2-3,112 his3-11 can1-100 carrying pNOY102 (GAL7-35S rDNA URA3)   [28]
MVY851 MATα rpa135::LEU2 ade2-1 ura3-1 trp1-1 leu2-3,112 his3-11 can1-100 carrying pNOY102 (GAL7-35S rDNA URA3) rpb1-1   This study
MVY858 HHYy168, MATα tor1-1 fpr1::NAT RPL13A-2xFKBP12::TRP1   [11]
MVY859 HHY170, MATα tor1-1 fpr1::NAT RPL13A-2xFKBP12::TRP1 RPO21-FRB::KANMX6   [11]
MVY860 MVY858 with RPA190-FRB::KANMX6   This study
MVY862 MVY858 with RPC160-FRB::KANMX6   This study
MVY868 MVY858 with TBP1-FRB::KANMX6   This study
MVY872 MVY859 with RPA190-FRB-HIS3MX   This study
MVY874 MVY859 with RPC160-FRB-HIS3MX   This study
MVY879 MVY872 with RPC160-FRB-LEU2   This study
CY1940 MVY858 with RPA190 FLAG Pol I AA This study
CY2032 MVY859 with RPA190 FLAG Pol I AA Pol II AA This study