Fig. 3
From: RNA polymerase II depletion promotes transcription of alternative mRNA species
Depletion of RNAPI but not RNAPIII induces the long RPB2 mRNA. a Nothern analysis [29] of the RPB2 mRNA isoforms after a RNAPI anchor-away (RPA190-FRB; MVY860), b RNAPIII anchor-away (RPC160-FRB; MVY862). c Northern analysis of the RPB2 mRNA isoforms after heat inactivation of RNAPII in the rpa135Δ rpb1-1 (MVY851). d Northern analysis of the RPB2 mRNA isoforms after RNAPIII anchor-away (RPC160-FRB; MVY862), except that cells are UV irradiated (70 J/m2) before adding rapamycin. e Northern analysis of the RPB2 mRNA isoforms after depletion of RNAPII and RNAPIII simultaneously (RPA190-FRB RPB1-FRB; MVY872)