Table 1 The eIF4E isoforms used throughout this study and primers used to clone the corresponding cDNA
From: Distinct recruitment of human eIF4E isoforms to processing bodies and stress granules
Gene | Protein isoform | Primer sequence | Restriction site | Protein variant (Acc. No./GI) | Encoded by transcript variant (Acc. No./GI): | ||
|---|---|---|---|---|---|---|---|
eIF4E1 | 1 | fwd | CGAAGAAGATCTATGGCGACTGTCGAACCGG | BglII | NP_001959.1 GI:4503535 | 1 | NM_001968.3 GI:194578905 |
rev | GCGCGGTACCTTAAACAACAAACCTATTTTTAGTGGTGG | Acc65I | |||||
3 | fwd | TTGTCGACATGTTGGACCTGACCTCCCGC | SalI | NP_001124150.1 GI:194578907 | 3 | NM_001130678.1 GI:194578906 | |
rev | GCGCGGTACCTTAAACAACAAACCTATTTTTAGTGGTGG | Acc65I | |||||
eIF4E2 | A | fwd | GTGTCGACATGAACAACAAGTTCGACGCTTTGAAAGATG | SalI | NP_004837.1 GI:4757702 | 1 | NM_004846.3 GI:545309374 |
rev | GGGTACCTCATGGCACATTCAACCGCGGCTTC | Acc65I | |||||
C | fwd | GTGTCGACATGAACAACAAGTTCGACGCTTTGAAAGATG | SalI | NP_001263265.1 GI:545309640 | 3 | NM_001276336.1 GI:545309639 | |
rev | CCGGTACCTCACAATGTGATTTTTGTATTTCGAAAGC | Acc65I | |||||
CRA_a | fwd | GTGTCGACATGAGTTTGAAAGATGATGAC | SalI | EAW71007.1 GI:119591413 | Â | not annotated yet | |
rev | CCGGTACCTCACAATGTGATTTTTGTATTTCGAAAGC | Acc65I | |||||
eIF4E3 | A* | fwd | CGGAGAAAATGGCGCTGCCC | X | NP_001128123.1 GI:197382708 | 1 | NM_001134651.1 GI:197382707 |
rev | ACTGTGGACGTGCTGTCCTTGG | X | |||||
B | fwd | GTGTCGACATGAGAGGAGAGAGGCGACCACTTTG | SalI | NP_775495.1 GI:27659734 | 2 | NM_173359.4 GI:197382627 NM_001134649.2 GI:544583504 NM_001134650.1 GI:197382663 NM_001282886.1 GI:544583491 | |
NP_001128121.1 GI:197382649 | 3 | ||||||
NP_001128122.1 GI:197382664 | 4 | ||||||
rev | CCGGTACCTTAGTGTTTTCCACGTCCACCTTCAAAAG | Acc65I | NP_001269815.1 GI:544583492 | 5 | |||