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Fig. 3 | BMC Molecular Biology

Fig. 3

From: Myelodysplasia-associated mutations in serine/arginine-rich splicing factor SRSF2 lead to alternative splicing of CDC25C

Fig. 3

Alternative splicing at the 3′UTR of endogenous SRSF2 transcripts. a Schematic of previously described SRSF2 isoforms (splice variants 3, 4, 5, and 6) that result from alternative splicing of the 3′UTR. Yellow arrows depict general location of primers used in RT-PCR. b Representative gel from RT-PCR of endogenous SRSF2′s 3′UTR after 48 h treatment with 2 μg/ml doxycycline. The sizes in base pairs of the PCR products corresponding to the splice variants are labeled on the right. c Bar graphs depicting changes in  % isoform 4 (v4) of total RNA. Statistics are based on two-way ANOVA with comparison to SRSF2WT (n = 4). Asterisks are pvalues as in Fig. 1; note that the data were compared to the WT and not to the tetON

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