Fig. 2

Apoptosis and subcellular localization of P95 and deletion SRSF2 mutants. a Representative immunofluorescence images of SRSF2HA cell lines (n = 3). CellMask Orange stained cytoplasm, DAPI stained DNA in the nucleus, anti-SC35 speckle marker stained SC35/SRSF2 in the nuclear speckles, anti-HA stained HA-tagged SRSF2 protein. b Average percentage of cells in early or late apoptosis at 48 h in the absence of doxycycline induction. c Average percentage of cells in early or late apoptosis at 48 h post-doxycycline induction. Statistics use two-way ANOVA with comparison to TF-1 TetON (n = 4). Asterisks are p values as in Fig. 1