Fig. 4
From: MicroRNA 433 regulates nonsense-mediated mRNA decay by targeting SMG5 mRNA
a, c Western Blot analysis of endogenous NMD substrates, TBL2 and GADD45B protein levels in cells 24 h after transfection with the miR-433 mimic (in BHK cells) or miR-433 inhibitor (in C2C12 cells) or a negative control miRNA mimic. β-actin was used as the internal control. b, d QPCR analysis of two NMD substrates, TBL2 and GADD45B relative mRNA expression level in cells 24 h after transfection with the miR-433 mimic (in BHK cells) or miR-433 inhibitor (in C2C12 cells) or a negative control miRNA mimic. *P < 0.05; **P < 0.01