Fig. 2

Knockdown SMG5 expression repressed NMD activity. a Quantitative polymerase chain reaction (qPCR) analysis of SMG5 mRNA expression in C2C12 cells transfected for 24 h with either three RNA interference fragments or negative control, positive control and Mock respectively. b Western Blot analysis of endogenous SMG5 protein levels in C2C12 cells 24 h after transfection with the RNA interference fragment or a negative control fragment. β-actin was used as the internal control. c–f showed the qPCR and western blot analysis of NMD substrates, TBL2 and GADD45B, mRNA and protein level in C2C12 cells 24 h after transfection with the RNA interference fragment or a negative control fragment. β-actin was used as the internal control. *P < 0.05; **P < 0.01