Fig. 1
From: MicroRNA 433 regulates nonsense-mediated mRNA decay by targeting SMG5 mRNA
MiR-433 targets the 3′-UTR of SMG5. a The predicated seed sequence in 3’-UTR of SMG5 by targetscan (http://www.targetscan.org/). b Luciferase expression level in HEK 293T cells transfected for 24 h with miR-433 mimic or a negative-control microRNA mimic. c Luciferase expression in Hela cells transfected for 48 h with miR-433 inhibitor or a negative-control inhibitor. d Luciferase expression level of the SMG5 WT vs SMG5 mutant in HEK 293T cells transfected for 24 h with a negative-control microRNA mimic (NC). *P < 0.05, and ** means the difference is significant, P < 0.01