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Fig. 6 | BMC Molecular Biology

Fig. 6

From: Stearoyl-CoA desaturase 1 expression is downregulated in liver and udder during E. coli mastitis through enhanced expression of repressive C/EBP factors and reduced expression of the inducer SREBP1A

Fig. 6

SREBP1a and C/EBPα exert their effects independently from each other. Reporter genes (1 µg) with the long or short promoter segment (PL, PS) were transfected into HepG2 or MAC-T cells together with 1000 ng of empty vector (−), 500 ng expressing C/EBPα or SREBP1a. Increasing amounts of vectors expressing either SREBP1a or C/EBPα (cf legend below figure) have in addition been co-transfected in other dishes. The total amount of transfected DNA was kept constant in all assays by eventually filling up with empty vector. RLUs/µg of protein were determined 48 h after transfection and normalized against the control, set as 1. Values are means from two experiments, each assayed in triplicate. Asterisks indicate statistical significance of difference relative to the control having not received any expression vector for transcription factors (*P < 0.05; **P < 0.001)

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BMC Molecular Biology

ISSN: 1471-2199

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