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Fig. 5 | BMC Molecular Biology

Fig. 5

From: Stearoyl-CoA desaturase 1 expression is downregulated in liver and udder during E. coli mastitis through enhanced expression of repressive C/EBP factors and reduced expression of the inducer SREBP1A

Fig. 5

Differentiation of the effect of C/EBPα and −β on the promoter activity. Increasing amounts of vectors (100–1000 ng) expressing C/EBPα (α, upper panels) or −β (β, lower panels) were transfected into HepG2 or MAC-T cultures harboring the long segment of the wt-promoter (black columns) or its variants with either the distal (striped columns) or both C/EBP binding sites mutated (checkerboard texture columns). Luciferase activity was recorded 48 h later and expressed as fraction of the control cultures with no C/EBP expression vector added (0 ng) set as 1. These assays were compared to the effect of increasing amounts of trans-dominant negative variants (DN-CEBP) of the respective C/EBP factors on the wt-promoter (open columns). Values are means from two experiments each assayed in triplicate. Asterisks indicate statistical significance of difference (*P < 0.05; **P < 0.01)

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BMC Molecular Biology

ISSN: 1471-2199

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