Fig. 3
From: Strong preference of BRCA1 protein to topologically constrained non-B DNA structures
Evidence of non-B DNA structures in pCFNO, pCMYC and pTA50 plasmids by nuclease cleavage. The plasmids pCFNO (lane 4), pCMYC (lane 6) and pTA50 (lane 5) have undergone cleavage by S1 nuclease with subsequent linearization by ScaI restriction endonuclease. Supercoiled pCFNO without any digestion (lane 2) and linearized pCFNO by ScaI (lane 3) without S1 cleavage were used as controls. Bands resulting from the nuclease S1 cleavage are about 1820 and 1167 bp. Lane 1 contains the 500 bp DNA ladder