Skip to main content
Fig. 2 | BMC Molecular Biology

Fig. 2

From: Strong preference of BRCA1 protein to topologically constrained non-B DNA structures

Fig. 2

BRCA1-L competition assay. a Competition gel shift assay. 5 pmol of labeled CF was incubated with 5 pmol of BRCA1-L and increasing amount of competitor non-labeled DNA. Competitor DNAs on the image are SS single strand, lane 3–7 and Q quadruplex, lane 10–14. CF/Competitor DNA ratios were 1:1 (lane 3, 10), 1:2 (lane 4, 11), 1:5 (lane 5, 12), 1:10 (lane 6, 13), 1:20 (lane 7, 14). Samples were incubated 15 min on ice in the binding buffer and then loaded onto an 8 % non-denaturating polyacrylamide gel and electrophoresed for 90 min at 4 °C. Arrow shows localization of the BRCA1-L/DNA complexes. Complexes without competitor DNA (lanes 2, 9). b Graph representation of the competition assay. The relative intensity of the BRCA1-L/DNA complexes are expressed as the percentage of the bands without competitor DNAs. Asterisks denote statistically significant difference (p < 0.05) of BRCA1-L biding to non-B DNA versus DS

Back to article page