Fig. 1
From: Strong preference of BRCA1 protein to topologically constrained non-B DNA structures
Comparison of BRCA1-L binding to different oligonucleotide structures. a Schematic representation of DNA substrates: SS single stranded, DS double stranded, Q quadruplex, CF cruciform, T triplex. b 5 pmol of labeled SS (lane 1–5), DS (lane 6–10), Q (lane 11–15), CF (lane 16–20), were incubated with increasing concentration of BRCA1-L (0/2.5/5/10/20 pmol) in the binding buffer (5 mM Tris–HCl, pH 7.0, 1 mM EDTA, 50 mM KCl and 0.01 % Triton X-100) for 15 min at 4 °C. Samples were electrophoresed on 8 % non-denaturating polyacrylamide gel at 100 V and 4 °C for 60 min