Fig. 4

Demonstration of DEspR protein and functionality in different human tumor cell lines by anti-DEspR mAbs. a FACs analysis of different cancer tissue type CSCs: glioblastoma, triple negative breast cancer (TNBC), non-small cell lung cancer (NSCLC), and pancreatic ductal adenocarcinoma (PDAC) using AF-568 labeled murine anti-DEspR mAbs compared with AF568-IgG2b murine isotype control. In Panc1 CSCs, low and high DEspR + CSCs are detected. b Multiple murine anti-DEspR mAbs (5g12e8, 7c5b2, 6g8g7) inhibit CSC growth in suspension culture determined by the number of live CSCs after 5 days of incubation with murine anti-DEspR mAbs, compared with corresponding control non-treated CSCs. Comparative analysis is presented using % change from respective controls; ***, One Way ANOVA with Tukey’s multiple comparisons test P < 0.0001. Epitope 1 murine mAbs: 5g12, 5g12e8, 7c5, 7c5b2; Epitope 2 murine mAbs: 6g8, 6g8e8