Fig. 7

zDHHC16 deficiency impaired the assembly/disassembly of DNA damage foci. Wild type and zDHHC16^−/− MEFs grown on coverslips were treated with 1 μM Dox for different periods of time. The cells were then fixed and immuno-stained for TopBP1 (a) and BRCA1 (c) using Texas-red conjugated secondary antibodies. The averaged numbers of foci under each condition were counted and averaged and presented at panels b and d, respectively. e zDHHC16 deficiency resulted in an increase in cell survival in response to Dox treatment. WT and zDHHC16^−/− MEFs were treated with 1 or 2 μM Dox for 24 h. Cell survival rates were determined by the Wst-1 assay. Asterisk denoted significant difference between wild type and zDHHC16^−/− MEFs