Table 1 Number of analyzed and validated KO clonal lines for the sgRNAs used
From: Immunoblot screening of CRISPR/Cas9-mediated gene knockouts without selection
SgRNA construct | # of clones tested by dot blot | # of validated KOs | KO percentage (%) |
|---|---|---|---|
HuR-3 | 69 | 15 | 22 |
HuR-4 | 22 | 8 | 36 |
HuR-5 | 157 | 9 | 6 |