Fig. 4From: Immunoblot screening of CRISPR/Cas9-mediated gene knockouts without selectionSequence of the genomic deletions in clone HuR-5.B5. The genomic region surrounding the targeted site was PCR-amplified from wt 293-TREx cells and clone HuR-5.B5, and placed into a plasmid vector. Individual plasmid clones were sequenced. The Cas9 cleavage site is denoted by a red triangle Back to article page